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lactase activity assay kit  (Elabscience Biotechnology)


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    Elabscience Biotechnology lactase activity assay kit
    Lactase Activity Assay Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lactase+assay+kit/Lactase+Activity+Assay+Kit/10__1016_slash_j__aninu__2026__06__005-96-19-46
    Average 91 stars, based on 10 article reviews
    lactase activity assay kit - by Bioz Stars, 2026-11
    91/100 stars

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    Related Articles

    Activity Assay:

    Article Title: Influence of Blastocystis hominis on the small intestine and lactase enzyme activity.
    Article Snippet: .. The amount of lactase in 1 mg of protein that hydrolyzes 1 nmol of lactose per minute at 37 C and pH 6.0 was defined as 1 unit according to the formula prescribed by the manufacture (Elabscience, 8th edition, Lactase Assay kit): Lactase activity U=mg protð Þ 1⁄4 ODSample ODControl ODS tan dard ODBlank Concentration of standard 5:55nmo=Lð Þ=Reaction time 20min :ð Þ =Protein concentration of sample mg prot mL 1000 ..

    Article Title: Aberrant Epithelial Differentiation Contributes to Pathogenesis in a Murine Model of Congenital Tufting Enteropathy
    Article Snippet: Following centrifugation at 3500xg for 10 min at 4°C, the supernatant was used for various assays and the protein content of the homogenate was measured using DC protein assay kit (Bio-Rad, Hercules, CA). .. Lactase activity was assessed using a Lactase Assay Kit (Cat# E-BC-K131-S, Elabscience, Houston, TX) according to the manufacturer’s instruction. .. Following centrifugation at 3500xg for 10 min at 4°C, the supernatant was used for various assays and the protein content of the homogenate was measured using DC protein assay kit (Bio-Rad, Hercules, CA).

    Article Title: Aberrant Epithelial Differentiation Contributes to Pathogenesis in a Murine Model of Congenital Tufting Enteropathy
    Article Snippet: Glucose concentration in blood was measured using a glucometer (Freestyle Lite, Abbott, Chicago, IL) and expressed as mg/dL. .. Lactase activity was assessed using a Lactase Assay Kit (Cat# E-BC-K131-S; Elabscience, Houston, TX) according to the manufacturer’s instruction. .. Alkaline phosphatase activity was assessed using an alkaline phosphatase fluorometric assay kit (ab83371, Abcam, Cambridge, United Kingdom) according to the manufacturer’s instruction.

    Concentration Assay:

    Article Title: Influence of Blastocystis hominis on the small intestine and lactase enzyme activity.
    Article Snippet: .. The amount of lactase in 1 mg of protein that hydrolyzes 1 nmol of lactose per minute at 37 C and pH 6.0 was defined as 1 unit according to the formula prescribed by the manufacture (Elabscience, 8th edition, Lactase Assay kit): Lactase activity U=mg protð Þ 1⁄4 ODSample ODControl ODS tan dard ODBlank Concentration of standard 5:55nmo=Lð Þ=Reaction time 20min :ð Þ =Protein concentration of sample mg prot mL 1000 ..



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    Influence of hydrogen peroxide–induced ROS on rat LSEC viability. Functional viability/reducing potential of the cell was assessed using resazurin/resorufin assay (A) and structural viability/cell integrity was studied with LDH-release assay (B). Measurements were conducted under continuous treatment with hydrogen peroxide on selected time points (0.5–5 h). The cell viability was calculated and normalized to the untreated control for resazurin assay and to the total LDH after cell lysis for LDH-release assay. Average data from 3 independent experiments/bioreplicates ±SD are presented. Statistical analyses below graphs show p values for trend analysis in time and concentration dependency. ANOVA for different times and concentrations compared to control values of nontreated cells; * p<0.05, ** p<0.01, *** p<0.001, ns non-significant. Abbreviations: LDH, lactase dehydrogenase; ROS, reactive oxygen species.

    Journal: Hepatology Communications

    Article Title: Hydrogen peroxide damage to rat liver sinusoidal endothelial cells is prevented by n -acetyl-cysteine but not GSH

    doi: 10.1097/HC9.0000000000000617

    Figure Lengend Snippet: Influence of hydrogen peroxide–induced ROS on rat LSEC viability. Functional viability/reducing potential of the cell was assessed using resazurin/resorufin assay (A) and structural viability/cell integrity was studied with LDH-release assay (B). Measurements were conducted under continuous treatment with hydrogen peroxide on selected time points (0.5–5 h). The cell viability was calculated and normalized to the untreated control for resazurin assay and to the total LDH after cell lysis for LDH-release assay. Average data from 3 independent experiments/bioreplicates ±SD are presented. Statistical analyses below graphs show p values for trend analysis in time and concentration dependency. ANOVA for different times and concentrations compared to control values of nontreated cells; * p<0.05, ** p<0.01, *** p<0.001, ns non-significant. Abbreviations: LDH, lactase dehydrogenase; ROS, reactive oxygen species.

    Article Snippet: LSECs were seeded on 48-well plates (300,000 cells/well), and a luminescence lactase dehydrogenase (LDH) detection kit (LDH-Glo, Promega) was used following the manufacturer’s instructions to assess cell viability.

    Techniques: Functional Assay, Lactate Dehydrogenase Assay, Control, Resazurin Assay, Lysis, Concentration Assay

    The effect of ROS-inducing versus ROS-depleting agents on rat LSEC viability, functions, and morphology. Cells were treated with ROS-inducing agents, hydrogen peroxide, or ROS-depleting agents: GSH or NAC. For cotreatments, samples were simultaneously treated with both hydrogen peroxide and GSH/NAC at the starting point of the assays. For pretreatments, samples were treated with GSH/NAC for 30 minutes, then rinsed with fresh media and treated with H 2 O 2 at the starting point of the assays. The functional (A) and structural (C) viability were studied using resazurin and LDH release assays, respectively; (B) intracellular ROS levels were assessed using a fluorescence-based ROS detection assay after 1 hour of treatment and (D) endocytic activity was measured with radiolabeled FSA-based scavenging assay during 2 hours of treatment. (E) LSEC morphology was quantitatively assessed using SEM images. Colors represent data from separate bioreplicates. Abbreviations: FSA, formaldehyde-treated serum albumin; GSH, glutathione; LDH, lactase dehydrogenase; NAC, n -acetyl cysteine; ROS, reactive oxygen species; SEM, scanning electron microscopy.

    Journal: Hepatology Communications

    Article Title: Hydrogen peroxide damage to rat liver sinusoidal endothelial cells is prevented by n -acetyl-cysteine but not GSH

    doi: 10.1097/HC9.0000000000000617

    Figure Lengend Snippet: The effect of ROS-inducing versus ROS-depleting agents on rat LSEC viability, functions, and morphology. Cells were treated with ROS-inducing agents, hydrogen peroxide, or ROS-depleting agents: GSH or NAC. For cotreatments, samples were simultaneously treated with both hydrogen peroxide and GSH/NAC at the starting point of the assays. For pretreatments, samples were treated with GSH/NAC for 30 minutes, then rinsed with fresh media and treated with H 2 O 2 at the starting point of the assays. The functional (A) and structural (C) viability were studied using resazurin and LDH release assays, respectively; (B) intracellular ROS levels were assessed using a fluorescence-based ROS detection assay after 1 hour of treatment and (D) endocytic activity was measured with radiolabeled FSA-based scavenging assay during 2 hours of treatment. (E) LSEC morphology was quantitatively assessed using SEM images. Colors represent data from separate bioreplicates. Abbreviations: FSA, formaldehyde-treated serum albumin; GSH, glutathione; LDH, lactase dehydrogenase; NAC, n -acetyl cysteine; ROS, reactive oxygen species; SEM, scanning electron microscopy.

    Article Snippet: LSECs were seeded on 48-well plates (300,000 cells/well), and a luminescence lactase dehydrogenase (LDH) detection kit (LDH-Glo, Promega) was used following the manufacturer’s instructions to assess cell viability.

    Techniques: Functional Assay, Fluorescence, Detection Assay, Activity Assay, Electron Microscopy